Recombinant Human FGF-19/FGF9 Protein (His Tag)(Active)

Sizes: 20μg, 100μg

Catalogue Numbers: PKSH032436-20, PKSH032436-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: FGF-19; FGF9

Target Symptom: Fibroblast growth factor 19; FGF-19; FGF19

Research Area: Signal Transduction; Neuroscience; Cancer; Stem cells

Target Species: Human

Expression Host: E.coli

Application: ELISA

Fusion Tag: N-6His

UNIProt ID: O95750

Accession: O95750

Background: Fibroblast growth factor 19 (FGF19) is a secreted protein which belongs to the FGFs family. FGF19 is expressed in fetal brain, cartilage, retina, and adult gall bladder. FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity. FGFRs can be important for regulation of glucose and lipid homeostasis. FGF19 has important roles as a hormone produced in the ileum in response to bile acid absorption. It has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. FGF19 can be considered as a regulator of energy expenditure.

Activity: Immobilized Human FGF-19-His at 2μg/ml(100 μl/well) can bind Human FGFR3-Fc(Cat: PKSH033678). The ED50 of Human FGF-19-His is 3. 1135 ug/ml.

Sequence: Phe27-Lys216

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 23.5 kDa

Observed MW: 24 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only